세포생물학 전체 MOC
📝 정리본 (강의노트 요약)
| 챕터 | 정리본 |
|---|---|
| Ch 9 | 000_Ch9_Visualizing Cells_정리본 |
| Ch 10 | 010_Ch10_Membrane_Structure_정리본 |
| Ch 12-1 | 000_Ch12-1_정리본 |
| Ch 12-2 | 000_Ch12-2_정리본 |
| Ch 13-1 | 000_Ch13-1_정리본 |
| Ch 13-2 | 000_Ch13-2_정리본 |
📚 기초 참고 자료
🔬 Ch 9. Visualizing Cells and Their Molecules
기초 개념
광학 현미경 (Optical Microscopy)
- optical microscope
- phase contrast microscopy(위상차 현미경)
- PSF(point spread function)
- 4 types of microscopy(시험예시 이미지)
형광 현미경 (Fluorescence Microscopy)
- 000_Fluorescence Microscopy(형광현미경)
- Confocal Microscope
- Multiphoton Microscopy
- TIRF(Total Internal Reflection Fluorescence Microscopy)
- deconvolution
형광 표지법
- GFP(Fluorescent Protein Tagging)
- Immunofluorescence Staining
- Fluorescent Biosensors for Cell Signaling
초고해상도 (Superresolution)
- SIM(Structured Illumination Microscopy)
- STED(Stimulated Emission Depletion)
- SMLM (Single-Molecule Localization Microscopy)
생체 내 단백질 동역학
전자 현미경 (Electron Microscopy)
- TEM(Transmission Electron Microscope)
- SEM(Scanning Electron Microscope)
- Cryo EM_Biological Specimen Preparation for Electron Microscopy
- Single Particle Reconstruction
- positively stained vs negatively stained
원자력 현미경
🧱 Ch 10. Membrane Structure
기초 개념
지질 (Lipids)
지질이중층 (Lipid Bilayer)
막과 연관된 구조
막 단백질 (Membrane Proteins)
- Membrane protein
- α-helical Conformation in Membrane Proteins
- β-Barrels Forming Large Channels
- Membrane bending protein
- Restricting domain of membrane protein
🗂 Ch 12. Intracellular Organization and Protein Sorting
개요
- Discovery of Cell and Organelle
- Biomolecular Condensates
- Protein Misfolding and Trafficking Disorders
막으로 둘러싸인 세포소기관
- 000_ Basic Set of Membrane‐enclosed Organelles in Eukaryotic Cells_MOC
- Prokaryotes and Eukaryotes
- Origin of Internal Membranes
- Biochemical Roles of Membranes
- Topological Relationships of Organelles
- Cytoplasm
- Endoplasmic Reticulum
- Mitochondria
- Nucleus
- Peroxisome
- chloroplasts
- Endosome
- Lysosome
단백질 수송 — ER (Protein Translocation)
- 000_Protein Traffic_MOC
- 001_Protein Translocation
- 002_Gated Transport
- 003_Vesicular Transport
- 004_Engulfment
- Sorting Signal
- SNARE Proteins
Co-/Post-translational Translocation
- Co-translational translocation
- Post-translational translocation
- Experimental basis for the signal hypothesis
관련 단백질/복합체
막 단백질 삽입 (Transmembrane Protein Insertion)
- Transmembrane Protein Insertion
- sequential use of cleaved ER signal and TM segment during membrane protein insertion
- Multipass Transmembrane Protein Processing
- Tailed-Anchored proteins insertion
단백질 변형·품질관리·수출
변형 (Modification)
폴딩 (Folding)
분해·UPR (Degradation)
- Quality control of ER protein folding
- Glycosylation-Based Monitoring
- Mannosidase Timer
- ER-associated degradation
- Unfolded Protein Response
막 합성 (Membrane Synthesis)
퍼옥시좀 (Peroxisome)
미토콘드리아 단백질 수입
- 000_Protein import into Mitochondria_MOC
- Translocators in mitochondrial membrane
- Transport into Matrix
- Transport into inner mitochondrial membrane
- 3 Energy source for import
- BAM pathway
핵공 복합체 (Nuclear Pore Complex)
핵 구조 (Nuclear Structure)
- 010_Nuclear structure_MOC
- Nuclear envelope
- Nuclear lamina
- Nucleolus
- Nuclear Pore Complex (structure)
NPC를 통한 수송
- 020_Transport through NPC_MOC
- Nuclear Localization Signals
- Nuclear Export Signals
- Nuclear Import Receptor
- Nuclear Export Receptor
- Ran-GTP cycle & Transport Through NPC
핵 수송 조절
- 021_Nuclear Transport Regulation_MOC
- Dorsal protein production control
- Nuclear import control during T-cell activation
🚚 Ch 13. Intracellular Membrane Traffic
소포 형성 (Vesicle Formation)
- Vesicle formation_MOC
- Clathrin coat
- AP2
- COPII
- Membrane Bending Protein
- Phosphatidylinositol(PI) and Phosphoinositides(PIPs)
- Coat Assembly Control by Monomeric GTPase
- Pinching-Off
- Nonspherical vesicle
소포 수송 메커니즘
소포 융합 (Vesicle Fusion)
ER-Golgi 수송
- Transportation from the ER Through the Golgi Apparatus_MOC
- Vesicular tubular cluster
- Retrieval Pathway to the ER
- CFTR
골지체 (Golgi Apparatus)
- Golgi apparatus_MOC
- functional compartmentalization of the Golgi apparatus
- Oligosaccharide processing in Golgi
- Proteoglycan Assembly
리소좀 (Lysosome)
세포내 이입 (Endocytosis)
- _endocytosis_MOC
- Receptor-mediated endocytosis
- Endosomes maturation
- ESCRT Protein Complex
- Caveolar endocytosis
- macropinocytosis
- phagocytosis
- Autophagy
분비 (Exocytosis)
- exocytosis_MOC
- secretory pathway
- direct sorting in TGN
- Proteolytical processing of secretory proteins
- synaptic vesicles
⚡ Ch 14. Energy Conversion in Mitochondria and Chloroplasts
기초 열역학
- Thermodynamics_MOC
- Spontaneous and non-spontaneous reactions
- Bond Energy
- Activation energy
- Enzyme reactions
- Substrate activation
에너지 대사 전체
해당과정 (Glycolysis)
- Glycolysis_MOC
- step 1 - hexokinase
- step 2 - phosphoglucose isomerase
- step 3 - phosphofructokinase
- step 4 - aldolase
- step 5 - triose phosphate isomerase
- step 6 - glyceraldehyde 3-phosphate dehydrogenase
- step 7 - phosphoglycerate kinase
- step 8 - phosphoglycerate mutase
- step 9 - enolase
- step 10 - pyruvate kinase
TCA 회로 (Citric Acid Cycle)
- Citric Acid Cycle_MOC
- step 1 - citrate synthase
- step 2 - aconitase
- step 3 - isocitrate dehydrogenase
- step 4 - alpha-ketoglutarate dehydrogenase
- step 5 - succinyl CoA synthetase
- step 6 - succinate dehydrogenase
- step 7 - fumarase
- step 8 - malate dehydrogenase
전자전달계 (Electron Transport Chain)
- Electron Transport Chain_MOC
- Complex I (NADH dehydrogenase)
- Complex II (Succinate dehydrogenase)
- Complex III (cytochrome c reductase)
- Complex IV (cytochrome c oxidase)
- Complex V (ATP synthase)
- proton pumping
- Chemiosmotic coupling
- ATP synthesis
지방산 β-산화 (Beta-Oxidation)
- beta-oxidation_MOC
- 1. Acyl CoA dehydrogenase
- 2. Enoyl CoA hydratase
- 3. Hydroxyacyl CoA dehydrogenase
- 4. B-Ketothiolase
미토콘드리아 유전학
- Mitochondria_MOC
- Endosymbiotic theory
- Mitochondrial DNA
- Mitochondrial Genetics
- Segregation of mitochondrial DNA
- mtDNA heteroplasmy
- Human diseases associated with mtDNA
골격근 (Skeletal Muscle)
🦴 Ch 16. The Cytoskeleton
개요
- Chemical Inhibitors of Actin and Microtubules
- Cytoskeleton during cell division
- Plectin
- SUN-KASH protein complexes
액틴 필라멘트 (Actin Filaments)
- 100_Actin filaments_MOC
- structures of an actin monomer and actin filament
- treadmilling
- major accessory proteins of the actin cytoskeleton
- actin arrays
- cell migration by actin cytoskeleton
- Rho family
미세소관 (Microtubules)
- 300_Microtubules_MOC
- structure of a microtubule
- preferential growth of microtubules
- Centrosome
- Microtubule organization in a neuron
운동 단백질 (Motor Proteins)
편모/섬모 (Flagellum / Cilium)
중간섬유 (Intermediate Filaments)
- 200_Intermediate filaments_MOC
- types of intermediate filament
- construction of intermediate filament
- Keratin filaments
골격근 세포 (Skeletal Muscle Cell)
- 400_Skeletal Muscle Cell_MOC
- Myosin II
- Myosin II cycle
- myofibrils
- Organization of accessory proteins in a sarcomere
- troponin
- T tubules
- sarcoplasmic reticulum
🔗 Ch 19. Cell Junctions and the Extracellular Matrix
세포 연접 — 세포-세포 (Cell-Cell Junctions)
- Cell Junctions_MOC
- Cell-Cell junction_MOC
- Homophilic vs heterophilic binding
- epithelial cells in the small intestine
Cadherin 기반 접착
- 00_Cadherin based adhension_MOC
- structure and function of cadherin
- cadherin-dependent cell sorting
- cadherin and catenin genetic diseases
Adherens Junction
- Adheres junction_MOC
- classical cadherin
- catenin
- initial formation of an adherens junction
- mechanotransduction in an adherence junction
Desmosome
Tight Junction
- 00_Tight junction_MOC
- adhension proteins in tight junction
- Function of tight junction
- tight junction genetic disorder
Gap Junction
세포 연접 — 세포-기질 (Cell-Matrix Junctions)
인테그린 (Integrin)
- integrin_MOC
- Types of Integrins
- Activation of Integrins by intracellular signaling
- Integrins in Cell-to-Cell interaction
- talin
- vinculin
- NCAM and ICAM
- Glanzmann Thrombasthenia
- Leukocyte Adhension Deficiency